Working some depths with my 100-400, 24-70, & 70-300mm lenses. ISO varies from 4000-20000. The 100-400mm f/4.5-5.6L IS USM is an awesome, clear & sharp lens that has an extremely tight focusing depth, which has 2 focusing options, auto & fine, with a manual focus lock. You can actually see shifts in the millimeter depth range in the viewfinder. My cat was the model, all shots indoors under normal lighting..
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